Determination of quinocide as impurity in primaquine tablets by capillary zone electrophoresis |
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Authors: | Abdalla A Elbashir Bahruddin Saad Abdussalam Salhin Mohamed Ali Muhammad Idiris Saleh Hassan Y Aboul‐Enein |
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Institution: | 1. School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia;2. Pharmaceutical and Medicinal Chemistry Department, National Research Centre, Tahrir Street, Dokki, Cairo 12311, Egypt |
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Abstract: | A capillary zone electrophoretic method has been developed and validated for the determination of the impurity quinocide (QC) in the antimalarial drug primaquine (PQ). Different buffer additives such as native cyclodextrins and crown ethers were evaluated. Promising results were obtained when either β‐cyclodextrin (β‐CD) or 18‐crown‐6 ether (18C6) were used. Their separation conditions such as type of buffer and its pH, buffer additive concentration, applied voltage capillary temperature and injection time were optimized. The use of 18C6 offers slight advantages over β‐CD such as faster elution times and improved resolution. Nevertheless, migration times of less than 5 min and resolution factors (Rs) in the range of 2–4 were obtained when both additives were used. The method was validated with respect to selectivity, linearity, limits of detection and quantitation, analytical precision (intra‐ and inter‐day variability) and repeatability. Concentrations of 2.12 and 2.71% (w/w) of QC were found in pharmaceutical preparations of PQ from two different manufacturers. A possible mechanism for the successful separation of the isomers is also discussed. Copyright © 2008 John Wiley & Sons, Ltd. |
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Keywords: | capillary electrophoresis primaquine quinocide β ‐cyclodextrin 18‐crown‐6 ethers |
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