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Azurin–Poly(N‐isopropylacrylamide) Conjugates by Site‐Directed Mutagenesis and their Thermosensitive Behavior in Electron‐Transfer Processes |
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| Authors: | Nadine Rosenberger Armido Studer Prof Dr Nobuyuki Takatani Dr Hiroshi Nakajima Dr Yoshihito Watanabe Prof Dr |
| Institution: | 1. Organisch‐Chemisches Institut, Westf?lische Wilhelms‐Universit?t, Corrensstrasse 40, 48149 Münster (Germany), Fax: (+49)?251‐83‐36523;2. Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa‐ku, 464‐8602 (Japan) |
| Abstract: | Searching for “intelligence” : Azurin–PNIPAM conjugates were prepared by site‐directed mutagenesis followed by protein reconstitution by using imidazole‐conjugated poly(N‐isopropylacrylamides). The polymer‐bound imidazole acts as a ligand in the active site of the blue copper protein azurin. The bioconjugates showed thermosensitive behavior in electron‐transfer processes with reduced cytochrome c.  |
| Keywords: | bioconjugates cytochrome enzymes point mutations polymers |