Key Laboratory of Medicine Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environment Science, Hebei University, Baoding 071002, Hebei Province (China), Fax: (+86)?312‐507‐9403
Abstract:
One‐nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling‐circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM , and the miRNA in a total RNA sample of a few nanograms can be determined.
