Department of Biochemistry and Biomedical Sciences and Department of Chemistry, McMaster University, 1200 Main Street West, Hamilton, ON L8N 3Z5 (Canada), Fax: (+1)?905‐522‐9033
Abstract:
Target detection by the naked eye : The action of an RNA‐cleaving allosteric DNAzyme in response to ligand binding was coupled to a rolling circle amplification process to generate long single‐stranded DNA molecules for colorimetric sensing (see scheme). Upon hybridization of the resulting DNA with a complementary PNA sequence in the presence of a duplex‐binding dye, the color of the dye changed from blue to purple.
