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Segmental Isotopic Labeling of a Central Domain in a Multidomain Protein by Protein Trans‐Splicing Using Only One Robust DnaE Intein |
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| Authors: | Alena E. L. Busche A. Sesilja Aranko Mehdi Talebzadeh‐Farooji Dr. Frank Bernhard Dr. Volker Dötsch Prof. Dr. Hideo Iwaï Dr. |
| Affiliation: | 1. Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance and Cluster of Excellence Frankfurt Macromolecular Complexes (CEF), University of Frankfurt, 60438 Frankfurt (Germany);2. Research Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, P.O. Box 65, Helsinki 00014 (Finland), Fax: (+358)?9‐1915‐9541 http://www.biocenter.helsinki.fi/bi/iwai/ |
| Abstract: | |
| Keywords: | inteins isotopic labeling NMR spectroscopy protein modifications protein splicing |