1. Materials Department, Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106 (USA), Fax: (+1)?805‐893‐8651;2. Department of Chemistry, University of California, Santa Barbara, Santa Barbara, CA 93106 (USA)
Abstract:
Singled out for its singularity : In a single‐step, single‐component, fluorescence‐based method for the detection of single‐nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self‐complementary DNA strand that forms a triple‐stem structure. The large conformational change that occurs upon binding to perfectly matched (PM) targets results in a significant increase in fluorescence (see picture; F=fluorophore, Q=quencher).
