Expression and Characterization of a Recombinant Truncated Capsid Protein of Hepatitis E Virus in Pichiapastoris |
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Authors: | YANG En-cheng CHI Bao-rong LI Xiao LIU Yan GAO Peng JIA Peng KAN Shi-fu WEN Zhong-mei WANG Wan JIN Ning-yi |
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Abstract: | Hepatitis E is an enterically transmitted viral disease caused by infection with hepatitis E virus(HEV).HEV is a nonenveloped virus that has been classified in the family of Caliciviridae.The virus appears to be a polyadenylated,positive-stranded RNA virus with three major open reading frames(ORFs).The capsid protein of HEV is encoded by the open reading frame 2(ORF2).We attempted to produce a truncated capsid protein,designed p293,in Pichia pastoris.The p293 gene encoding amino acids(aa) 382-674 of HEV ORF... |
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Keywords: | Hepatitis E virus Capsid protein Pichia pastoris Protein purification |
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