| DMPC与蛋白质在气-液界面上复合组装过程研究 |
| |
| 引用本文： | 张恒建,陈志坚,毕只初,王明强,翟秀红,崔光晨,李峻柏. DMPC与蛋白质在气-液界面上复合组装过程研究[J]. 化学学报, 2000, 58(9): 1125-1130 |
| |
| 作者姓名： | 张恒建 陈志坚 毕只初 王明强 翟秀红 崔光晨 李峻柏 |
| |
| 作者单位： | 中国科学院化学研究所,分子科学中心胶体与界面科学实验室,北京,100101 |
| |
| 基金项目： | 中国科学院资助项目，国家杰出青年科学基金，，29925307，， |
| |
| 摘 要： | 对磷脂DMPC与蛋白质(β-lactoglobulin，β-casein或humanserumalbumin)组成的复合单分子膜在压缩过程中的相变进行了研究，同时通过Brewster角显微镜观察了磷脂与蛋白质在空气-水界面处发生的自组装过程。发现当低表面压时在DMPC/β-casein，DMPC/humanserumalbumin和DMPC/β-lactoglobulin复合单分子膜上分别出现线状和块状微区，而在较高表面压时只有DMPC/β-casein体系出现钩状的微区。说明微区的形貌与蛋白质的种类及构象变化有关。
|
| 关 键 词： | 蛋白质 磷脂 单分子膜 自组装 相变 Brewster角显微镜 |
| 修稿时间： | 2000-01-24 |
The self-assembly process of DMPC/protein at the air/water interface |
| |
| ZHANG Hen-Jian,CHEN Zhi-Jian,BI Zhi-Chu,WANG Ming-qiang,ZHAI Xiu-Hong,CUI Guang-Chen,LI Jun-Bai. The self-assembly process of DMPC/protein at the air/water interface[J]. Acta Chimica Sinica, 2000, 58(9): 1125-1130 |
| |
| Authors: | ZHANG Hen-Jian CHEN Zhi-Jian BI Zhi-Chu WANG Ming-qiang ZHAI Xiu-Hong CUI Guang-Chen LI Jun-Bai |
| |
| Affiliation: | Inst of Chem, CAS.Beijing(100080) |
| |
| Abstract: | The phase changes of the complex monolayer composed of DMPC and β- lactoglobulin or β-casein during the compression process were investigated. The self-assembly process of the phospholipid and the protein at the air/water interface was observed via Brewster angle microscopy. It was found that at the low surface pressure during the compress process the linear or piece shape domain appeared with the DMPC/β-casein or the DMPC/β-lactoglobulin complex monolayer, respectively. At the higher surface pressure, the hook shape domains were formed for the DMPC/β-casein system. |
| |
| Keywords: | Brewster angle microscopy (BAM) phospholipid protein complex monolayer self- assembly |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《化学学报》浏览原始摘要信息 |
|
点击此处可从《化学学报》下载全文 |
