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A novel fluorescent guanine derivative distinguishable of three structures, single strand, duplex, and quadruplex |
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| Authors: | Akimitsu Okamoto Keiichiro Kanatani Yoshio Saito |
| Affiliation: | a Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan b SORST, Japan Science and Technology Corporation, Kyoto 615-8510, Japan c NEWCAT Institute, Faculty of Engineering, Nihon University, Tamura, Koriyama 963-8642, Japan |
| Abstract: | We have constructed a pyrene-labeled guanine base, 8PyG. 8PyG is a novel fluorescent probe for monitoring the secondary structure of G-rich DNA. The fluorescence emitted from 8PyG-labeled oligodeoxynucleotide clearly distinguished three structural states, single strand-duplex-quadruplex. Thus, the technique, which monitors the fluorescence of 8PyG-labeled oligodeoxynucleotide, is a powerful tool for the investigation of DNA structural changes. |
| Keywords: | Pyrene Oligodeoxynucleotide Fluorescence Quadruplex |
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