New reagent for the preparation of oligonucleotides involving a 5′-thiophosphate or a 5′-phosphate group |
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Authors: | Rémy Lartia |
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Institution: | Centre de Biophysique Moléculaire CNRS, UPR 4301 affiliated with the University of Orléans and with INSERM, FR 2708, Rue Charles Sadron, 45071 Orléans Cedex, 02 France |
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Abstract: | We report here a novel, simple reagent enabling the chemical incorporation of a thiophosphate or a phosphate group at the 5′-end of oligonucleotides using very mild basic deprotection conditions. This method can be useful in the case of alkali sensitive modified oligonucleotides. This reagent also gives access to the preparation of bifunctional oligonucleotides with either a thiophosphate group at the 5′-end and a phosphate at the 3′-end, or two thiophosphate groups at both the 5′- and the 3′-ends, or a 5′-thiophosphate group and a 3′-amino-containing linker. |
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Keywords: | New phosphorylating reagent 5&prime -Phosphorylated oligonucleotides 5&prime -Thiophosphorylated oligonucleotides Mild deprotection conditions |
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