Fluorescence-based multiplex protein detection using optically encoded microbeads |
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Authors: | Jun Bong-Hyun Kang Homan Lee Yoon-Sik Jeong Dae Hong |
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Institution: | School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Korea. |
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Abstract: | Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding. |
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