| 菌紫质中视黄醛超快异构化反应的动态光谱分析 |
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| 引用本文： | 吴义室,钟声,艾希成,胡坤生,张建平. 菌紫质中视黄醛超快异构化反应的动态光谱分析[J]. 高等学校化学学报, 2008, 29(1): 140-143 |
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| 作者姓名： | 吴义室 钟声 艾希成 胡坤生 张建平 |
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| 作者单位： | 中国科学院化学研究所,北京分子科学国家实验室,分子动态与稳态结构化学国家重点实验室,北京,100080;中国人民大学理学院化学系,北京,100872;中国科学院生物物理研究所,北京,100101;中国人民大学理学院化学系,北京,100872 |
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| 摘 要： | 利用飞秒时间分辨吸收光谱方法研究了菌紫质(BR)光循环中视黄醛超快异构化反应过程. 发展了结合全局拟合的奇异值分解(SVD)分析方法, 建立了超快异构化反应动力学模型, 解析了几个重要的中间态I460, J625和K590的物种相关差异光谱(SADS)和布居动力学, 确定了光致异构化反应过程. 同时明确了700 nm附近存在的受激荧光来自于弗兰克-康登跃迁态(H中间态)的贡献, 其衰减寿命为0.04 ps. 这些结果对深入认识H态在超快异构化反应过程中的作用具有参考价值.
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| 关 键 词： | 菌紫质 超快异构化 奇异值分解 受激发射 |
| 文章编号： | 0251-0790（2008）01-0140-04 |
| 收稿时间： | 2007-05-30 |
| 修稿时间： | 2007-05-30 |
Time-Resolved Spectral Analysis of Ultrafast Isomerization Dynamics of Retinal in Bacteriorhodopsin |
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| WU Yi-Shi,ZHONG Sheng,AI Xi-Cheng,HU Kun-Sheng,ZHANG Jian-Ping. Time-Resolved Spectral Analysis of Ultrafast Isomerization Dynamics of Retinal in Bacteriorhodopsin[J]. Chemical Research In Chinese Universities, 2008, 29(1): 140-143 |
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| Authors: | WU Yi-Shi ZHONG Sheng AI Xi-Cheng HU Kun-Sheng ZHANG Jian-Ping |
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| Affiliation: | Beijing National Laboratory for Molecular Science(BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China;;Department of Chemistry, College of Science, Renmin University of China, Beijing 100872, China;;Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | The ultrafast photoisomerization process of retinal in Bacteriorhodpsin's(BR)photocycle was investigated via femtosecond time-resolved absorption spectroscopy.Singular value decomposition(SVD)combined with model-based global fitting was employed to analyze the time-resolved spectra,and the related difference spectra(SADS)and the population dynamics of I460,J625 and K590 intermediates were derived on the basis of our proposed reaction scheme.The detailed photoisomerization pathways were discussed.Moreover,the SADS of Franck-Condon state(H intermediate),radiative transition of which accounts for the stimulated emission around 700 nm,was also detected,and its time constant was 0.04 ps.Our results prove the involvement of the H-intermediate in ultrafast photoisomerization process of retinal in BR. |
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| Keywords: | Bacteriorhodopsin Ultrafast isomerization Singular value decomposition Stimulated emission |
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