On-line coupling of packed column supercritical fluid chromatography with multiple wavelength detector and radioactivity flow-through detector

Summary The coupling of packed column supercritical fluid chromatography with a commercial Radioactivity Flow-Through Detector for the sensitive and highly selective detection of radioactive nuclides (e.g.¹⁴C is described. The radioactivity flow-through detector showed no baseline shift when pressure or modifier (methanol) gradients were applied. The detector cell was pressure resistant even at high flow rates, over a period of approx. 100 hours and showed no leakage problems even at 40°C, 340 bar, 4.0 ml/min and a modifier content up to 20%.