十六羧酸基酞菁锌光敏剂与白蛋白相互作用的光谱研究及复合物的制备

利用光谱法研究了光敏剂分子2,3,9,10,16,17,23,24-八(3,5-二羧酸基苯氧基)酞菁锌(ZnPc(COOH)₁₆)与白蛋白(BSA和HSA)的相互作用，结果表明，ZnPc(COOH)₁₆与BSA，HSA存在较强的相互作用，结合常数为2.25～2.94×106 L·mol-1。同时，通过温育摩尔比为2∶1的ZnPc(COOH)₁₆和白蛋白混合物，进而凝胶色谱分离纯化，得到了摩尔比为1∶1的ZnPc(COOH)₁₆-BSA和ZnPc(COOH)₁₆-HSA复合物。光谱研究表明，ZnPc(COOH)₁₆在复合物中以单体形式存在，Q带最大吸收峰和发射峰均较单纯的ZnPc(COOH)₁₆有所红移。

Spectroscopic Studies on the Interactions of a Hexadeca-Carboxy Zinc Phthalocyanine and Serum Albumin and Preparation of the Bioconjugates

The interactions of the new photosensitizer {2, 3, 9, 10, 16, 17, 23, 24-octakis[(3,5-dicarboxy)phenoxy]-phthalocyaninato}zinc(II) [ZnPc(COOH)16] and serum albumins (BSA and HSA) were investigated by absorption and fluorescence spectroscopy. The binding constants were found to be 2.25-2.94 x 10(6) L x mol(-1). The 1 : 1 ZnPc (COOH)16-BSA and ZnPc (COOH)16-BSA conjugates were also prepared by incubating a mixture of ZnPc(COOH)16 and the corresponding serum albumin in 2 : 1 mole ratio followed by separation by gel permeation chromatography. The Q band absorption and fluorescence emission of ZnPc(COOH)16 are slightly red-shifted in the conjugates, and the spectral features suggest that the phthalocyanine exists predominantly in monomeric form in the conjugates.