A Rationally Designed Thymidine‐Based Self‐Assembled Monolayer on a Gold Electrode for Electroanalytical Applications |
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Authors: | Dr Dhrubajyoti Datta Dr Raj Kumar Bera Saibal Jana Bhaskar Manna Debayan Roy Prof Anakuthil Anoop Prof C Retna Raj Prof Tanmaya Pathak |
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Institution: | 1. Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, India;2. (+91)?3222‐255303 |
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Abstract: | A self‐assembled monolayer (SAM) of 1‐(3,5‐epidithio‐2,3,5‐trideoxy‐β‐d ‐threo‐pentofuranosyl)thymine (EFT) on a gold electrode was prepared and characterized by Raman spectral and electrochemical measurements. Voltammetric and electrochemical impedance measurements show that the SAM of EFT on a Au electrode impedes the electron‐transfer reaction. The SAM of EFT was successfully used for the voltammetric sensing of urate in neutral solution. The coexisting ascorbate anion does not interfere and therefore the EFT‐based electrode was able to quantify urate at the micromolar level in the presence of a large excess amount of ascorbate. To demonstrate the practical applications, the amount of urate in two different human serum samples was quantified by using the EFT‐based electrode; the results are in good agreement with those determined by the clinical method. DFT calculations show that both ascorbate and urate have noncovalent interactions including hydrogen‐bonding interactions with EFT. |
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Keywords: | gold hydrogen bonds monolayers self-assembly sensors |
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