Measurement of binding kinetics between PI3-K and phosphorylated IGF-1R using a surface plasmon resonance biosensor |
| |
Authors: | Xueling Li Chi-chun Fong Minghui Huang Huimin Cao Jianlong Zhao Mengsu Yang |
| |
Institution: | (1) State key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China |
| |
Abstract: | A high-performance amperometric glucose biosensor was developed, based on immobilization of glucose oxidase (GOx) on a copper
(Cu) nanoparticles/chitosan (CHIT)/carbon nanotube (CNT)-modified glassy carbon (GC) electrode. The Cu and CNT had a synergistic
electrocatalytic effect toward the reduction of hydrogen peroxide in the matrix of biopolymer CHIT. The Cu/CHIT/CNT modified
GC electrode could amplify the reduction current of hydrogen peroxide greatly. Besides, the Cu/CHIT/CNT modified GC electrode
reduces hydrogen peroxide at a much lower applied potential and inhibit the responses of interferents. With GOx as an enzyme
model, a new glucose biosensor was fabricated. The sensitivity of the sensor is due not only to the large microscopic area
but also to the high efficiency of transformation of H2O2 generated by enzymatic reaction to current signal. The biosensor exhibited excellent sensitivity (the detection limit is
down to 0.02 mM), fast response time (less than 4 sec), wide linear range (from 0.05 to 12 mM), and perfect selectivity.
Correspondence: Wanzhi Wei, State key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering,
Hunan University, Changsha 410082, China |
| |
Keywords: | : Copper carbon nanotubes chitosan glucose oxidase biosensor |
本文献已被 SpringerLink 等数据库收录! |
|