Detection of 19-nortestosterone and its urinary metabolites in miniature pigs by gas chromatography-mass spectrometry |
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| Authors: | G Debruyckere C Van Peteghem |
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| Institution: | Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, State University of Ghent, Belgium. |
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| Abstract: | The metabolism of 19-nortestosterone was investigated in a miniature non-castrated male pig (boar), in a castrated pig (barrow) and in a female pig (sow). Urine samples were taken before and at regular intervals after the injection of 100 mg of Laurabolin (nortestosterone laurate). The sample clean-up consists in preliminary solid-phase extraction, followed by high-performance liquid chromatographic purification and fractionation. Finally, gas chromatography-mass spectrometry is used to identify the 19-nortestosterone metabolites. |
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