基于金属纳米粒子增敏的SPR生物传感器检测吲哚乙酸

本实验建立了表面等离子体共振(SPR)生物传感器检测3-吲哚乙酸(IAA)的方法。制备了两种SPR生物传感器检测IAA:传统模式的SPR生物传感器1和Au/Ag合金纳米粒子增敏的SPR生物传感器2。结果发现:传感器1在IAA浓度范围为175~350μg/L时,浓度与其波数位移值呈线性关系,检出限为25μg/L(S/N=3);传感器2在IAA浓度范围为17.5~250μg/L时,浓度与其波数位移值呈线性关系,检出限为2.2μg/L(S/N=3)。说明基于Au/Ag合金纳米粒子的传感器2比传感器1有较高的灵敏度和较低的检出限。加标回收实验测得加标回收率范围为96%~100.2%,平均值为98.4%。本实验制备的SPR生物传感器具有较好的精密度、稳定性、重现性和特异性。

Determination of Indole Acetic Acid by SPR Biosensor Based on the Metal Nanoparticles Sensitization

Surface plasmon resonance (SPR) biosensor was a new method for detecting 3-indole acetic acid (IAA).We adopted two kinds of indole acetic acid SPR biosensors to detect IAA:Traditional sensor 1 and Au/ Ag alloy nanoparticles based sensor 2.Sensor 1:The wavenumber shifts were linearly proportional to the IAA concentration in a range from 175 to 350 μg/L with a detection limit of 25 μg/L (S/N=3).Sensor 2:The wavenumber shifts were linearly proportional to the IAA concentration in a range from 17.5 to 250 μg/L with a detection limit of 2.2 μg/L (S/N =3).The results showed that the sensor 2 based on Au/Ag alloy nanoparticles had higher sensitivity and lower detection limit than the the sensor 1.The rate of standard addition recovery was 96％-100.2％,the average was 98.4％.The prepared SPR biosensor had good precision,stability,repeatability and specificity.