Identification of human esterase D subunits from the homodimeric and heterodimeric forms of five phenotypes by a new two-dimensional isoelectric focusing method |
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| Authors: | A Alonso G Visedo M Sancho J Fernández-Piqueras |
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| Affiliation: | Seccion de Biologia, Instituto Nacional de Toxicologia, Madrid, Spain. |
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| Abstract: | A new two-dimensional isoelectric focusing method was developed to identify the human esterase D (EsD) subunits from the homodimeric and heterodimeric forms of five EsD phenotypes. EsD polymorphism was also analyzed by one-dimensional isoelectric focusing under reducing and mild denaturing conditions to study the influence of dithiothreitol and low concentrations of urea on the focusing pattern of the EsD dimers. |
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