| pBPB共价修饰蝮蛇酸性磷脂酶A2的晶体学数据 |
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| 引用本文： | 赵海燕,宋时英,林政炯,杜晓燕,周元聪.pBPB共价修饰蝮蛇酸性磷脂酶A2的晶体学数据[J].物理化学学报,1997,13(5):449-451. |
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| 作者姓名： | 赵海燕 宋时英 林政炯 杜晓燕 周元聪 |
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| 作者单位： | National Laboratory of Biomacromolecules,Institute of Biophysics,Academia Sinica,Beijing 100101,Shanghai Institute of Biochemistry,Academia Sinica,Shanghai 200031 |
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| 摘 要： |
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| 关 键 词： | 磷脂酶A2 pBPB抑制剂 晶体生长 X-射线衍射 |
| 收稿时间： | 1996-08-21 |
| 修稿时间： | 1997-01-06 |
The Crystallization of Acidic Phospholipase A_2 from the Venom of Agkistrodon Blomhoffii Brevicaudus Covalently Inhibited by p-Bromo-phenacyl-bromide |
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| Zhao Hai-Yan,Song Shi-Ying,Lin Zheng-Jiong,Du Xiao-Yan,Zhou Yuan-Cong.The Crystallization of Acidic Phospholipase A_2 from the Venom of Agkistrodon Blomhoffii Brevicaudus Covalently Inhibited by p-Bromo-phenacyl-bromide[J].Acta Physico-Chimica Sinica,1997,13(5):449-451. |
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| Authors: | Zhao Hai-Yan Song Shi-Ying Lin Zheng-Jiong Du Xiao-Yan Zhou Yuan-Cong |
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| Institution: | National Laboratory of Biomacromolecules,Institute of Biophysics,Academia Sinica,Beijing 100101|Shanghai Institute of Biochemistry,Academia Sinica,Shanghai 200031 |
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| Abstract: | The Crystals of acidic phospholipase A2 from the venom of Agkistrodon blomhoffii brevicaudus (i.e. Agkistrodon halys pallas) covalently modified with p-bromo-phenacylbromide were obtained by the method of hanging drop vapor diffusion. Crystallization droplet was composed of 0.06mol?L-1 Na(CH3)2AsO2 (pH=6.5), 24.8% (v/v) 1,4-butanediol, and 4.5mg?mL-1 protein. The crystal data are of a=b=82.82?., c=32.85?, space group P61. 8945 unique reflections with 1.93 ? resolution were measured on a Siemens area detector X-ray diffractometor, of which 8069 reflections having F0 >2σ(F). The experimental results show that the crystals are suitable to a structure analysis of high resolution. |
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| Keywords: | Phospolipase A2 pBPB inhibition Crystallization X-ray diffraction |
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