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| 干细胞迁移机理的近场扫描光学显微术研究 | |
| 引用本文： | 陈家楠,潘运龙,丁晖,覃莉,蔡继业. 干细胞迁移机理的近场扫描光学显微术研究[J]. 分析测试学报, 2010, 29(6). DOI: 10.3969/j.issn.1004-4957.2010.06.004 |
| 作者姓名： | 陈家楠 潘运龙 丁晖 覃莉 蔡继业 |
| 作者单位： | 1. 暨南大学,生命科学技术学院,生物纳米技术实验室,广东,广州,510632 2. 暨南大学,附属第一医院普外科,广东,广州,510632 3. 暨南大学,医学院,组织胚胎学教研室,广东,广州,510632 |
| 基金项目： | 国家自然科学基金资助项目，"973计划"资助项目 |
| 摘 要： | 将内皮细胞生长因子(VEGF)置于甲基纤维素碟中形成VEGF的浓度梯度分布,并将人脐带间充质干细胞(Mesenchymal stem cells,MSCs)于此浓度梯度中培养,观察VEGF能否诱导MSCs定向迁移。应用近场扫描光学显微术(Near-field scanning optical microscopy,NSOM)同时获取了VEGF诱导前后的MSCs的形貌和光学信息。结果表明,近场光学图观测到形貌图上所没有的黑色斑点,分析认为这些黑斑为细胞的黏着斑。近场光学图显示经过VEGF诱导后细胞的黏着斑数量明显增加。同时,对诱导前后干细胞的骨架蛋白进行免疫荧光标记并用共聚焦显微镜进行观察,结果表明细胞骨架由诱导前的无序状态转变为诱导后的有序状态,说明诱导后的干细胞处于迁移状态。光学超微结构图则显示了诱导后干细胞表面的光学细节比诱导前细胞大量增加,出现了大量直径约200 nm的光斑,这是由于细胞表面大量分泌黏附分子等蛋白分子引起的,这些结果为VEGF能够诱导MSCs进行定向迁移提供了实验依据和可视化证明。也表明NSOM不但能提供高分辨的光学分辨率,还可提供生物细胞精细结构的更深层次的光学信息。 |
| 关 键 词： | 近场光学显微镜 间充质干细胞 黏着斑 |
Study on Migration of Mesenchymal Stem Cells by Near-field Optical Microscopy |
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| CHEN Jia-nan,PAN Yun-long,DING Hui,QIN Li,CAI Ji-ye. Study on Migration of Mesenchymal Stem Cells by Near-field Optical Microscopy[J]. Journal of Instrumental Analysis, 2010, 29(6). DOI: 10.3969/j.issn.1004-4957.2010.06.004 | |
| Authors: | CHEN Jia-nan PAN Yun-long DING Hui QIN Li CAI Ji-ye |
| Affiliation: | CHEN Jia-nan1,PAN Yun-long2,DING Hui2,QIN Li3,CAI Ji-ye1(1.Lab of Bio-nanotechnology,College of Life Science and Technology,Jinan University,Guangzhou 510632,China,2.Department of Surgery,The First Affiliated Hospital,3.Department of Fistology and Embryology,Medical College,China) |
| Abstract: | To investigate whether vascular endothelial growth factor(VEGF) could induce human mesenchymal stem cells(MSCs) to migrate directionally,methyl cellulose discs imbued with VEGF were placed in a dish to generate a concentration gradient of VEGF.MSCs were then placed in a given area with a defined distance from the methyl cellulose discs.Near-field scanning optical microscopy(NSOM) was used to obtain simultaneously the morphological and optical informations of MSCs before and after VEGF induction.Results show... |
| Keywords: | near-field optical scanning microscopy(NSOM) mesenchymal stem cells(MSCs) adhesion plaque |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |