Determination of medroxyprogesterone acetate residues by CE immunoassay with chemiluminescence detection |
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| Authors: | Peng Chi-Fang Huo Tie-Ming Liu Li-Qiang Chu Xiao-Gang Xu Chuan-Lai |
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| Affiliation: | School of Food Science and Technology, Southern Yangtze University, WuXi, JiangSu Province, PR China. |
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| Abstract: | A rapid and simple method is developed for the determination of medroxyprogesterone acetate (MPA) by CE immunoassay with chemiluminescence (CL). This method is based on the competitive reactions between horseradish peroxidase (HRP)-labeled MPA (MPA-HRP) and free MPA with anti-MPA antiserum. The influencing factors on the electrophoresis and CL detection were studied completely and the optimal conditions of separation and determination were obtained. The linear range was 2.0-50 nmol/L and the LOD for MPA was 0.9 nmol/L. The present method was applied to the analysis of pork tissues. |
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| Keywords: | CE Chemiluminescence Immunoassay Medroxyprogesterone acetate |
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