Simultaneous determination of MHD and DMD in dog plasma by high-performance liquid chromatography with fluorescence detection and its application to pharmacokinetic studies |
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Authors: | Xiaoyu Li Guangji Wang Haitang Xie Rui Wang Meijuan XU Wei Wang Jining Tao Jianguo Sun |
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Institution: | (1) Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, 24# Tong Jia Xiang Street, 210, Nanjing, 210009, People’s Republic of China;(2) Shanghai Fudan-Zhangjiang Bio-pharmaceutical Co. Ltd., Shanghai, 201203, People’s Republic of China |
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Abstract: | A rapid, reproducible high-performance liquid chromatographic (HPLC) method with fluorescence detection for the simultaneous
determination of 3(or 8)-(1-methoxyethyl)-8(or 3)-(1-hydroxyethyl)-deuteroporphyrin IX (MHD) and 3,8-di-(1-methoxyethyl)-deuteroporphyrin
IX (DMD) in dog plasma was described. Fluorescein was used as an internal standard. A simple extraction step with ethyl acetate
was performed before chromatography on a Diamonsil C18 column (5 μm, 4.6 mm×150 mm). The chromatography used 0.02 mol L−1 sodium acetate/tetrahydrofuran (66:34 v/v). The analytical curve was linear over the concentration range 0.025– 2.5 μg mL−1. For a 100 μL dog plasma sample, the limit of determination for both MHD and DMD was 0.025 μg mL−1. The recoveries of MHD and DMD were more than 76% and 89%, respectively. The intra-assay (within-run) and interassay (between-run)
coefficients of variation (precisions) for MHD and DMD were less than 15%. This method was found to be suitable for the analysis
of biosamples and was successfully applied to pharmacokinetic studies of Deuxemether in dogs. |
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Keywords: | Deuxemether MHD DMD HPLC Pharmacokinetics |
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