| 石墨炉原子吸收光谱法测定血清中非蛋白结合铜和锌 |
| |
| 引用本文： | 张源,林哲绚,李伟秋,张俏忻,李慧,罗红军,罗文鸿. 石墨炉原子吸收光谱法测定血清中非蛋白结合铜和锌[J]. 光谱学与光谱分析, 2009, 29(10): 2864-2866. DOI: 10.3964/j.issn.1000-0593(2009)10-2864-03 |
| |
| 作者姓名： | 张源 林哲绚 李伟秋 张俏忻 李慧 罗红军 罗文鸿 |
| |
| 作者单位： | 汕头大学医学院中心实验室,广东,汕头,515041;汕头大学医学院中心实验室,广东,汕头,515041;汕头大学医学院中心实验室,广东,汕头,515041;汕头大学医学院中心实验室,广东,汕头,515041;汕头大学医学院中心实验室,广东,汕头,515041;汕头大学医学院中心实验室,广东,汕头,515041;汕头大学医学院中心实验室,广东,汕头,515041 |
| |
| 基金项目： | 国家自然科学基金，广东省自然科学基金 |
| |
| 摘 要： | 建立两步沉淀蛋白质-石墨炉原子吸收法测定血清中非蛋白结合铜和锌的方法。无水乙醇以2∶1与血清混合后，再经70 ℃温度变性处理，可将蛋白质完全沉降。 用氘灯校正背景，石墨炉原子吸收法进行测定，方法检测限：Cu，1.2 μg·L-1，Zn，0.098 μg·L-1。回收率：Cu，92.3%～104%， Zn，为90%～107%。 利用此方法测定了肿瘤患者血清中游离铜与锌，并与健康人作了对比。
|
| 关 键 词： | 铜 锌 GFAAS 蛋白质沉淀 |
| 收稿时间： | 2008-05-06 |
Determination of Trace Free Copper and Zinc in Serum Samples by Graphite Furnace Atomic Absorption Spectrometry after Two-Step Precipitation of Protein |
| |
| ZHANG Yuan,LIN Zhe-xuan,LI Wei-qiu,ZHANG Qiao-xin,LI Hui,LUO Hong-jun,LUO Wen-hong. Determination of Trace Free Copper and Zinc in Serum Samples by Graphite Furnace Atomic Absorption Spectrometry after Two-Step Precipitation of Protein[J]. Spectroscopy and Spectral Analysis, 2009, 29(10): 2864-2866. DOI: 10.3964/j.issn.1000-0593(2009)10-2864-03 |
| |
| Authors: | ZHANG Yuan LIN Zhe-xuan LI Wei-qiu ZHANG Qiao-xin LI Hui LUO Hong-jun LUO Wen-hong |
| |
| Affiliation: | Central Laboratories, Shantou University Medical College, Shantou 515041, China |
| |
| Abstract: | A method was developed for the determination of trace free copper and zinc in serum sample by GFAAS after two-step precipitation. The serum and ethanol were mixed with volume ratio of 1:2. The mixture was subsequently denatured at 70 ℃ and centrifuged to precipitate proteins. The determination, limit of copper (3σ) was 1.2μg ·L^-1 and the recovery was 92. 3%- 104%, and the determination. Limit of zinc(3σ) was 0. 098μg ·L^-1 and the recovery was 90%-107%. This two-step precipitation method can be used to determine non-protein-bound copper in tumor patients and healthy people. |
| |
| Keywords: | GFAAS |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《光谱学与光谱分析》浏览原始摘要信息 |
|
点击此处可从《光谱学与光谱分析》下载全文 |
|
