| 高效液相色谱法测定烟叶提取物中茄尼醇的含量 |
| |
| 引用本文： | 张明时,黄俊学.高效液相色谱法测定烟叶提取物中茄尼醇的含量[J].色谱,2001,19(5):470-471. |
| |
| 作者姓名： | 张明时 黄俊学 |
| |
| 作者单位： | 贵州师范大学分析测试中心 |
| |
| 摘 要： | 采用硅胶色谱柱 ,以正己烷 异丙醇 (体积比为 98∶2 )混合液为流动相 ,在紫外检测波长设定为 2 15nm的高效液相色谱仪上测定了烟叶提取物中茄尼醇的含量。实验结果表明 :方法在茄尼醇进样量为 1μg～ 10 μg时有良好线性关系 (Y =16 6 2 0 4X - 32 5 3,r=0 9997) ;加标回收实验 (n =6 )的平均回收率为 98 1% ,RSD为 1 9% ;方法简便 ,有良好的精密度和准确性。
|
| 关 键 词： | 高效液相色谱法 茄尼醇 烟叶提取物 |
| 文章编号： | 1000-8713(2001)05-0470-02 |
| 修稿时间： | 2001年1月21日 |
Determination of Solanesol in the Extracts of Tobacco Leaves by High Performance Liquid Chromatography |
| |
| ZHANG Ming shi,HUANG Jun xue.Determination of Solanesol in the Extracts of Tobacco Leaves by High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2001,19(5):470-471. |
| |
| Authors: | ZHANG Ming shi HUANG Jun xue |
| |
| Institution: | Center for Analysis and Test, Guizhou Normal University, Guiyang 550001, China. zhangmingshi@263.net |
| |
| Abstract: | A method for the determination of solanesol in the extracts of tobacco leaves by high performance liquid chromatography has been developed. The column used was Shim-pack CLC-SIL(5 microns, 5 mm i.d. x 150 mm) and the UV detector was set at 215 nm. The eluent was a mixture of n-hexane and isopropyl alcohol (98:2, V/V). As the results showed, the linear range was 1 microgram-10 micrograms(r = 0.9997), the average recovery of solanesol was 98.1% and the relative standard deviation was 1.9%. This method is fast, accurate and reproducible. |
| |
| Keywords: | high performance liquid chromatography solanesol tobacco extract |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《色谱》浏览原始摘要信息 |
| 点击此处可从《色谱》下载免费的PDF全文 |
