Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins |
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| Authors: | Xu Chenjie Xu Keming Gu Hongwei Zhong Xiaofen Guo Zhihong Zheng Rongkun Zhang Xixiang Xu Bing |
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| Institution: | Department of Chemistry, Department of Physics, and Bioengineering Program, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China. chbingxu@ust.hk |
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| Abstract: | Using Nalpha,Nalpha-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt-NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins. |
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