Synthesis of a new Pt(II) complex containing valganciclovir drug and calf-thymus DNA interaction study using multispectroscopic methods |
| |
Authors: | Nahid Shahabadi Somaye Fatahi Maryam Maghsudi |
| |
Institution: | 1. Faculty of Chemistry, Department of Inorganic Chemistry, Razi University, Kermanshah, Iran;2. Medical Biology Research Center (MBRC), Kermanshah University of Medical Sciences, Kermanshah, Iran |
| |
Abstract: | A new complex, Pt(valcyte)(DMSO)Cl]Cl, in which valcyte (trade name) served as valganciclovir hydrochloride drug (2-(2-amino-6-oxo-3H-purin-9-yl)methoxy]-3-hydroxypropyl](2S)-2-amino-3-methylbutanoate), was synthesized and characterized by different physicochemical methods. Binding interaction of this complex with calf-thymus DNA (ct-DNA) has been investigated by multispectroscopic techniques. The complex displays significant binding properties with ct-DNA. The results of fluorescence and UV–vis absorption spectroscopy indicated that this complex interacted with ct-DNA in a groove-binding mode, and the binding constant was 3.8 × 104 M?1. Furthermore, the complex induced detectable changes in the CD spectrum of ct-DNA and slightly changed its viscosity which verified the groove-binding mode. Finally, all results indicated that Pt(II) complex interact with DNA via groove-binding mode. |
| |
Keywords: | Valganciclovir hydrochloride platinum complex DNA binding circular dichroism Hoechst 33258 groove-binding |
|
|