| Flow—injection Chemiluminescence Determination of Reserpine in Medicine and Biological Fluids with Controlled—Reagent—Release Technology |
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| 引用本文： | 宋正华,张尼.Flow—injection Chemiluminescence Determination of Reserpine in Medicine and Biological Fluids with Controlled—Reagent—Release Technology[J].中国化学,2003,21(2):175-180. |
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| 作者姓名： | 宋正华 张尼 |
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| 作者单位： | DepartmentofChemistry,NorthwestUniversity,Xi’an,Shaanxi710069,China |
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| 基金项目： | ProjectsupportedbytheNaturalScienceFoundationofEducationCommittee，ShaanxiProvince，China . |
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| 摘 要： | A sensitive and rapid chemiluminescence(CL) flow injection with controlled-reagent-release technology for the determination of reserpine was proposed.The Cl reagents,luminol and dichromate,uses in this sensor,were all immobilized on anion-exchange resin.Through injection of 100μl of water,the reagents on the anion-ex-change resin column were eluted and in the presence of reserpine ,the CL intensity was decreased,by which reserpine could be sensed.Reserpine was quantified by measuring the decrement of CL intensity,which was observed linear with the logrithm of reserpine concentration in the rage of 1.0-500.0ng/mL,and the limit of detection was 0.4ng/mL(3σ)with a relative standard deviation of less than 3.0?The proposed procedure was applied in the assay of reserpine in pharmaceutical preparation and biological fhuids without any pre-treatment process and with sampling frequencies of 72 times per hour.
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| 关 键 词： | 利血平 流动注射化学发光测定法 药物分析 生物体液 尿液 |
Flow-injection Chemiluminescence Determination of Reserpine in Medicine and Biological Fluids with Controlled-Reagent-Release Technology |
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| Song Zheng‐Hua,Zhang Ni.Flow-injection Chemiluminescence Determination of Reserpine in Medicine and Biological Fluids with Controlled-Reagent-Release Technology[J].Chinese Journal of Chemistry,2003,21(2):175-180. |
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| Authors: | Song Zheng‐Hua Zhang Ni |
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| Abstract: | A sensitive and rapid chemiluminescence (CL) flow injection with controlled‐reagent‐release technology for the determination of reserpine was proposed. The CL reagents, luminol and dichromate, used in this sensor, were all immobilized on anion‐exchange resin. Through injection of 100 μL of water, the reagents on the anion‐exchange resin column were eluted and in the presence of reserpine, the CL intensity was decreased, by which reserpine could be sensed. Reserpine was quantified by measuring the decrement of CL intensity, which was observed linear with the logarithm of reserpine concentration in the range of 1.0–500.0 ng/mL, and the limit of detection was 0.4 ng/mL (3σ) with a relative standard deviation of less than 3.0%. The proposed procedure was applied in the assay of reserpine in pharmaceutical preparation and biological fluids without any pre‐treatment process and with sampling frequencies of 72 times per hour. |
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| Keywords: | reserpine flow injection chemiluminescence medicine urine |
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