Improving Liquid Chromatography-Mass Spectrometry Sensitivity Using a Subambient Pressure Ionization with Nanoelectrospray (SPIN) Interface |
| |
Authors: | Keqi Tang Jason S Page Ioan Marginean Ryan T Kelly Richard D Smith |
| |
Institution: | (1) Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA; |
| |
Abstract: | In this work, the subambient pressure ionization with nanoelectrospray (SPIN) ion source and interface, which operates at
~15–30 Torr, is demonstrated to be compatible with gradient reversed-phase liquid chromatography-MS applications, exemplified
here with the analysis of complex samples (a protein tryptic digest and a whole cell lysate). A low liquid chromatographic
flow rate (100–400 nL/min) allowed stable electrospray to be established while avoiding electrical breakdown. Efforts to increase
the operating pressure of the SPIN source relative to previously reported designs prevented solvent freezing and enhanced
charged cluster/droplet desolvation. A 5- to 12-fold improvement in sensitivity relative to a conventional atmospheric pressure
nanoelectrospray ionization (ESI) source was obtained for detected peptides. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|