| 反相高效液相色谱法测定生物转化体系中的甘草酸 |
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| 引用本文： | 李晖,卢定强,刘伟民.反相高效液相色谱法测定生物转化体系中的甘草酸[J].色谱,2004,22(3):258-259. |
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| 作者姓名： | 李晖 卢定强 刘伟民 |
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| 作者单位： | 1. 南京工业大学制药与生命科学学院,江苏,南京,210009
2. 江苏大学生物与环境工程学院,江苏,镇江,212013 |
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| 基金项目： | 国家重点基础研究专项经费资助(项目号:2003CB716006). |
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| 摘 要： | 采用高效液相色谱法在Hypersil C18色谱柱(4.6 mm i.d.×250 mm,5 μm)上以甲醇-水-冰醋酸(70∶30∶1, 体积比)为流动相分离测定了甘草酸单铵盐生物(酶)转化体系中的甘草酸,流动相流速为1.0 mL/min,紫外检测波长254 nm。实验结果表明,该方法在进样量为0.2~20 μg时具有良好的线性；样品的加标回收率为98%～103％,相应的相对标准偏差为0.16％～1.58％。方法简便、快速、可靠。
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| 关 键 词： | 甘草酸 高效液相色谱法 生物(酶)转化体系 |
| 文章编号： | 1000-8713(2004)03-0258-02 |
| 修稿时间： | 2003年7月21日 |
Determination of Glycyrrhizinic Acid in Biotransformation System by Reversed-Phase High Performance Liquid Chromatography |
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| LI Hui,LU Dingqiang,LIU Weimin.Determination of Glycyrrhizinic Acid in Biotransformation System by Reversed-Phase High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2004,22(3):258-259. |
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| Authors: | LI Hui LU Dingqiang LIU Weimin |
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| Institution: | College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China. |
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| Abstract: | A method for determining glycyrrhizinic acid in the biotransformation system by reversed-phase high performance liquid chromatography (RP-HPLC) was developed. The HPLC conditions were as follows: Hypersil C18 column (4.6 mm i.d. x 250 mm, 5 microm) with a mixture of methanol-water-acetic acid (70:30:1, v/v) as the mobile phase; flow rate at 1.0 mL/min; and UV detection at 254 nm. The linear range of glycyrrhizinic acid was 0.2-20 microg. The recoveries were 98%-103% with relative standard deviations between 0.16% and 1.58% (n = 3). The method is simple, rapid and accurate for determining glycyrrhizinic acid. |
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| Keywords: | high performance liquid chromatography glycyrrhizinic acid biotransformation system |
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