Real-time multicolor DNA detection with chemoresponsive diffraction gratings and nanoparticle probes |
| |
Authors: | Bailey Ryan C Nam Jwa-Min Mirkin Chad A Hupp Joseph T |
| |
Institution: | Department of Chemistry and Institute for Nanotechnology, Northwestern University, Evanston, Illinois 60208, USA. |
| |
Abstract: | We report a real-time DNA detection method that utilizes single-strand DNA-modified nanoparticle probes and micropatterned chemoresponsive diffraction gratings interrogated simultaneously at multiple laser wavelengths. The surface-bound nanoparticle probe based assay with the chemoresponsive diffraction grating signal transduction scheme results in an experimentally simple DNA detection protocol, displaying attributes of both detection methodologies: the high sensitivity and selectivity afforded by nanoparticle probes and the experimental simplicity, wavelength-dependent resonant enhancement features, and miniaturization potential provided by the diffraction-based sensing technology. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|