PNA-Modified Magnetic Nanoparticles and Their Hybridization with Single-Stranded DNA Target: Surface Enhanced Raman Scatterings Study |
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| Authors: | Feng Wang Hebai Shen Jie Feng Haifeng Yang |
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| Institution: | (1) College of Life and Environment Science, Shanghai Normal University, 200234 Shanghai, China |
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| Abstract: | A 4-pyridyldithiol-derivatized peptide nucleic acid (PNA), which was designed to recognize a specific gene, was attached to
3-mercapropropyloxysilane coated magnetic nanoparticles (MNPs) via a thiol-disulfide exchange reaction. Subsequently, PNA-functionalized
magnetic nanoparticles (PMNPs) were prepared. Sequentially the PMNPs were challenged with non-complementary and perfect-match
DNA targets. The entire procedure was monitored using surface-enhanced Raman scattering (SERS). The results showed that the
PMNPs were able to efficiently hybridize with the perfect-match ssDNA target and showed no affinity towards non-complementary
DNA. This approach may provide a means of direct and label-free gene analysis. |
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| Keywords: | : Peptide nucleic acid (PNA) magnetic nanoparticles (MNPs) PNA-modified MNPs (PMNPs) surface-enhanced Raman scattering (SERS) |
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