纳米电极时空分辨监测单个PC12细胞多巴胺量子释放

细胞作为有机体结构与生命活动的基本单位 ,在生命体内的新陈代谢和信息传递等方面起着关键作用 .而细胞受激释放是其参与生命活动的形式之一 ,在整个生命活动中起着非常重要的作用 ,不正常的细胞释放会导致生物体功能紊乱 ,以致产生各种疾病 .对细胞释放进行研究在神经生物学、生物化学、临床、病理和药学等多个学科领域中都具有非常重要的意义1] .由于细胞的超微体积以及胞内单个囊泡量子释放发生的时间是毫秒级的 ,因此需要一种快速灵敏检测的手段才能对胞内释放情况进行实时监测 .对于多巴胺、肾上腺素、去甲肾上腺素及五羟色胺等具有…

Spatial and Temporal Amperometric Monitoring Dopamine Release from Single PC12 Cells with Carbon Fiber Nanoelectrode

Carbon fiber nanoelectrodes(200-300 nm) were firstly used to amperometricaly monitor the dopamine release from single PC12 cells with temporal resolution and especially more higher spatial resolution than those obtained by using microelectrodes. When the nanoelectrode was in a distance 1 μm above the PC12 cell, only one peak signal corresponding to a single vesicle exocytotic event was detected caused from the stimulation with 1 mmol/L nicotine. The spatial difference of exocytosis was also detected by placing the electrode onto the different locations of the cell body, the results have demostrated that the spatial distribution of dopamine in cells is not uniform and the time for stimulating secretion is very different. Nanoelectrodes electrochemical method can provide a powerful tool for observing the temporal and spatial characteristics of the secretion from single cells directly.