Histidine as a dipolar eluent in ion chromatography of aliphatic amines |
| |
Authors: | P Hajós K Horváth R Conca C Sarzanini |
| |
Institution: | (1) Department of Analytical Chemistry, University of Veszprém, P.O.B. 158, 8201, Hungary;(2) Department of Analytical Chemistry, University of Torino, 10121 Via Giuria 5, Italy |
| |
Abstract: | Summary Acidified L-histidine was found to be a suitable eluent in membrane-suppressed cation chromatography for the separation of
aliphatic mono-amines (methyl, ethyl, trimethyl, triethyl, tripropylamines). Based on the protonation equilibrium of amines
with the histidine eluent, a simplified separation method was developed, in which the eluent can act as a source of diprotic
cations and analytes as monoprotic amines. On detection, the suppressor converts the eluent by deprotonation to its dipolar
form with minimum conductivity at the pH of the isoelectric point of histidine, (pl 7.56). Efficient separations were observed
under isocratic development with an eluent concentration of CHIS≤2 mM and a pH below 2.0. The effect of eluent concentration and number of carbon atoms in the aliphatic chain on the retention
and the resolution was determined. Selectivity data of the separation system are also presented.
Presented at Balaton Symposium '01 on High-Performance Separation Methods, Siófok, Hungary, September 2–4, 2001 |
| |
Keywords: | Column liquid chromatography Ion chromatography Suppressed conductivity detection Underivatized aliphatic amines Dipolar (zwitterionic) eluent components |
本文献已被 SpringerLink 等数据库收录! |
|