Study of normal colorectal tissue by FT-Raman spectroscopy |
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Authors: | P O Andrade R A Bitar K Yassoyama H Martinho A M E Santo P M Bruno A A Martin |
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Institution: | 1.Laboratory of Biomedical Vibrational Spectroscopy, Institute of Research and Development (IP&D),Universidade do Vale do Paraiba (UniVap),S?o José dos Campos, SP,Brazil;2.Vivalle Hospital,S?o José dos Campos, SP,Brazil |
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Abstract: | FT-Raman spectroscopy was employed to study normal human colorectal tissues in vitro with the aim of evaluating the spectral
differences of the complex colon mucous in order to establish a characteristic Raman spectrum. The samples were collected
from 39 patients, providing 144 spectra for the statistical analysis. The results enable one to estabilish three well-defined
spectroscopic groups of non-altered coloretal tissues that were consistently checked by statistical (clustering) and biological
(histopathology) analyses: group 1 is represented by samples with the presence of epithelial layer, connective tissue papillae,
and smooth muscle tissue; group 2 comprises tissues with epithelial layer and connective tissue papillae; group 3 presented
mostly fatty and slack conjunctive tissue. The study reveals the existence of an intrinsic spectral variability for each patient
that must be considered when sampling tissues fragments to build a spectral database. This is the first step for future studies
and applications of Raman spectroscopy to optical biopsy and diagnosis of colorectal cancer. |
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Keywords: | FT-Raman spectroscopy Colorectal Cancer Optical biopsy |
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