The optimization of capacity and efficiency when coupling fused silica open tubular columns in gas chromatography |
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Authors: | R. E. Murphy R. R. Heath J. G. Dorsey |
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Affiliation: | (1) Agriculture Research Service Insect Attractants, Behavior and Basic Biology Research Laboratory, United States Department of Agriculture, 32604 Gainesville, FL, USA;(2) Department of Chemistry, University of Florida, 32611 Gainesville, FL, USA;(3) Analytical Research, Rohm and Haas Company, 727 Norristown Rd., 19477 Spring House, PA, USA;(4) Department of Chemistry, University of Cincinnati, 45221-0172 Cincinnati, OH, USA |
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Abstract: | Summary There are a number of parameters which have to be chosen depending on the analysis being done in gas chromatography. While the choice of stationary phase material is based on the solutes to be separated, the thickness is dependent on the concentration and the volatility of the components to be analyzed. This study undertakes a coupled column phase ratio optimization by connecting a short piece of a particular column prior to a normal length of an analytical column. Various columns of different dimensions (phase ratio), but of the same stationary phase material (methyl silicone), are coupled together by a deactivated glass press-fit connector, and the efficiency and capacity are measured. The coupling of fused silica open tubular columns is optimized in efficiency by matching or decreasing the phase ratio of successive columns. Capacity optimization is accomplished by increasing the phase ratio of consecutive columns. Capacity and efficiency optimization are opposing each other; therefore, if some efficiency can be sacrificed a substantial increase in capacity is possible. |
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Keywords: | Gas chromatography Fused silica open tubular columns Serial coupled columns Phase ratio optimization |
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