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QuEChERS method for the simultaneous quantification of phorate and its metabolites in porcine and chicken muscle and table eggs using ultra‐high performance liquid chromatography with tandem mass spectrometry
Authors:Tae Woong Na  Young‐Jun Lee  Joon‐Seong Park  Amer Ramadan  Han Sol Lee  Hyung Suk Chung  Jeong‐Heui Choi  Ho‐Chul Shin  Jae‐Han Shim
Institution:1. Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea;2. Jeonnam Bioindustry Foundation, Bio Control Research Center Immyeon, Gokseonggun, Jeollanamdo, Republic of Korea;3. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;4. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea
Abstract:An analytical method to detect phorate and its metabolites, including phorate sulfone, phorate sulfoxide, phoratoxon, phoratoxon sulfone, and phoratoxon sulfoxide, in porcine and chicken muscles and table eggs was developed and validated. Extraction was performed using a quick, easy, cheap, effective, rugged, and safe method and analysis was conducted using ultra‐high performance liquid chromatography‐tandem mass spectrometry. Matrix‐matched calibrations were linear over the tested concentrations, with determination coefficient ≥ 0.995 for all tested analytes in the different matrices. The limits of detection and quantification were 0.001 and 0.004 mg/kg, respectively. The calculated recovery rates at three fortification levels were satisfactory, with values between 74.22 and 119.89% and relative standard deviations < 10%. The method was applied successfully to commercial samples collected from locations throughout the Korean Peninsula, and none of them showed any traces of the tested analytes. Overall, the developed method is simple and versatile, and can be used for monitoring phorate and its metabolites in animal products rich in protein and fat.
Keywords:Chicken muscle  Metabolites  Phorate  Porcine muscle  Table eggs
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