Efficient fabrication of high‐capacity immobilized metal ion affinity chromatographic media: The role of the dextran‐grafting process and its manipulation |
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Authors: | Qiang Li Rongyue Zhang Kai Zhu Jia Suo Zhiguo Su Zhigang Zhang Guanghui Ma |
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Affiliation: | 1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, PR China;2. Department of Chemical Engineering, Beijing Institute of Petro‐chemical Technology, Beijing, PR China;3. School of Chemical & Environmental Engineering, China University of Mining & Technology, Beijing, PR China;4. Hebei Normal University of Science & Technology, Qinhuangdao, Hebei, PR China |
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Abstract: | Novel high‐capacity Ni2+ immobilized metal ion affinity chromatographic media were prepared through the dextran‐grafting process. Dextran was grafted to an allyl‐activated agarose‐based matrix followed by functionalization for the immobilized metal ion affinity chromatographic media. With elaborate regulation of the allylation degree, dextran was completely or partly grafted to agarose microspheres, namely, completely dextran‐grafted agarose microspheres and partly dextran‐grafted ones, respectively. Confocal laser scanning microscope results demonstrated that a good adjustment of dextran‐grafting degree was achieved, and dextran was distributed uniformly in whole completely dextran‐grafted microspheres, while just distributed around the outside of the partly dextran‐grafted ones. Flow hydrodynamic properties were improved greatly after the dextran‐grafting process, and the flow velocity increased by about 30% compared with that of a commercial chromatographic medium (Ni Sepharose FF). A significant improvement of protein binding performance was also achieved by the dextran‐grafting process, and partly dextran‐grafted Ni2+ chelating medium had a maximum binding capacity for His‐tagged lactate dehydrogenase about 2.5 times higher than that of Ni Sepharose FF. The results indicated that this novel chromatographic medium is promising for applications in high‐efficiency and large‐scale protein purification. |
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Keywords: | Dextran Immobilized metal ion affinity chromatography Nickel Protein purification |
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