亲和色谱中配基的筛选与应用

亲和配基的选择与筛选是发展新的亲和色谱填料或构建一个新的亲和色谱体系所必须解决的首要问题。该文结合作者所在实验室的工作,对配基的选择、筛选与应用方面的一些进展进行了简要评述。作者所在实验室针对特定蛋白质和多肽的多肽亲和配基的筛选,开展了反义肽简并性的研究,发展了基于反义肽的组合化学筛选新方法。与常规的组合合成法相比,该方法简单、快捷、有效,极大地减小了合成和筛选的工作量,降低了筛选后亲和组分结构鉴定的难度。所建立的筛选策略已应用于流感病毒、严重急性呼吸道综合征(SARS)病毒亲和抑制剂的筛选和用于人β-干扰素测定的石英晶体微天平（QCM）生物传感器的构建,均取得了有意义的结果。

Ligand Screening in Affinity Chromatography and Its Applications

Affinity chromatography is one of the most powerful techniques in selective purification and isolation of a great number of compounds. Affinity ligand is the most important factor in affinity chromatography, therefore, the selection and the screening of affinity ligand become the first issue to be considered when a novel type of affinity packing or a novel system of affinity chromatography needs to be developed. In combination with the research work of the author' s group, this paper briefly summaries the recent progresses in selection, screening and application of affinity ligands. A new screening method with antisense peptide based combinatorial peptide libraries has been developed to search the affinity ligands for target proteins and peptides. This strategy is simpler and more effective than the conventional combinatorial strategy. The work including syntheses and screening is greatly reduced and the structural determination of the ligands becomes easy by using this method. Furthermore, the new method has been successfully applied to the screening of inhibitors for influenza A virus and SARS coronavirus, as well as the construction of quartz crystal microbalance ( QCM) biosensor for human interferon-beta.