Fluorimetric reaction detectors for high-performance liquid chromatography Part 2. Reactor materials and effects of variable dynamic dimensions |
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| Authors: | G. Schwedt E. Reh |
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| Affiliation: | (1) Fachbereich 8-Analytische Chemie, Universität-Gesamthochschule Siegen, Adolf-Reichwein-Str. 2, D-5900 Siegen 21, Federal Republic of Germany;(2) Present address: Anorg.-Chem. Institut der Universität, Tammanstr. 4, D-3400 Göttingen, FRG |
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| Abstract: | Summary Using selected steroid pairs some effects of the coupling of liquid chromatographic separations with derivatization after separation on band broadening are investigated. Derivatization with isonicotinoylhydrazid in organic solvents and the multi-step reaction of ketosteroids to form dihydrolutidin derivatives in aqueous solution are given as examples. The dependence of the resolution on the reactor materal, on the design of the reactor, on the linear velocity of the liquid stream in the reactor and on the ratio of the flow rate in the HPLC column and the reactor are examined.Part 1. Selection of Chemical reactions |
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| Keywords: | Reaction detector HPLC Fluorimetry Reactor materials Coupling conditions |
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