Interaction of imidazoline- and imidazolidine-based nitroxides with chloroplasts |
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| Authors: | V. V. Ptushenko L. N. Ikryannikova I. A. Grigor’ev I. A. Kirilyuk B. V. Trubitsin A. N. Tikhonov |
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| Affiliation: | 1. Department of Biophysics, Faculty of Physics, Moscow State University, M. V. Lomonosov Moscow State University, 119992, Moscow, Russia
2. Institute of Organic Chemistry, Russian Academy of Sciences, Novosibirsk, Russian Federation
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| Abstract: | In this work, we investigated the interaction of a number of novel pH-sensitive spin probes (imidazoline- and imidazolidine-based nitroxides) with isolated chloroplasts. The light-induced changes in the electron paramagnetic resonance signals of these radicals are determined by several factors: ΔpH-driven accumulation of nitroxides inside the thylakoids, consumption (or evolution) of oxygen by chloroplasts, and irreversible loss of spin probe paramagnetism. A comparative study of these radicals characterized by different pKa allowed us to conclude that these spin probes can be used as pH indicators for monitoring the intrathylakoid pH- and light-induced changes in oxygen concentration in chloroplast suspension. |
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