Rapid amplification of genetically modified organisms using a circular ferrofluid-driven PCR microchip |
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Authors: | Yi Sun Yien-Chian Kwok Peter Foo-Peng Lee and Nam-Trung Nguyen |
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Institution: | (1) National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore, 637616, Singapore;(2) School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore |
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Abstract: | The use of genetically modified organisms (GMOs) as food and in food products is becoming more and more widespread. Polymerase
chain reaction (PCR) technology is extensively used for the detection of GMOs in food products in order to verify compliance
with labeling requirements. In this paper, we present a novel close-loop ferrofluid-driven PCR microchip for rapid amplification
of GMOs. The microchip was fabricated in polymethyl methacrylate by CO2 laser ablation and was integrated with three temperature zones. PCR solution was contained in a circular closed microchannel
and was driven by magnetic force generated by an external magnet through a small oil-based ferrofluid plug. Successful amplification
of genetically modified soya and maize were achieved in less than 13 min. This PCR microchip combines advantages of cycling
flexibility and quick temperature transitions associated with two existing microchip PCR techniques, and it provides a cost
saving and less time-consuming way to conduct preliminary screening of GMOs.
Figure Schematic of the circular ferrofluid-driven PCR microchip |
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Keywords: | Genetic modified organism (GMO) Ferrofluid PCR microchip PMMA |
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