HPLC determination of plasma taurine |
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Authors: | P. A. Biondi F. Manca A. Negri C. Lucarelli |
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Affiliation: | (1) Istituto di Fisiologia Veterinaria e Biochimica, Via Celoria 10, 20133 Milano, Italy;(2) Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy |
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Abstract: | Summary The taurine content in plasma was determined by high performance liquid chromatographic analysis of its dansyl derivative with fluorometric detection. After the reaction with dansyl chloride, the derivative was extracted from an aqueous mixture by using tetrabutylammonium as a counter-ion. The influences of different tetrabutylammonium salts and of the eluent mixture composition were studied. Omotaurine, added as the internal standard to the plasma samples, assured good reproducibility. The procedure was applied to the determination of taurine in specimens from different mammalian species. |
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Keywords: | Taurine Column liquid chromatography Plasma Ion-pair extraction |
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