具有内皮细胞选择性的细胞膜仿生支架材料的研究

利用AIBN引发自由基反应,由单体2-(甲基丙烯酰氧基)乙基-2-(三甲基氨基)乙基磷酸酯(MPC)、甲基丙烯酸十八酯(SMA)、对硝基苯氧羰基聚乙二醇甲基丙烯酸酯(MEONP)合成了一种新型类细胞膜仿生涂层材料.MPC可以阻抗非特异性吸附;MEONP可以结合抗体或多肽促进特异性识别.通过表面固定的方法引入多肽序列Arg-Glu-Asp-Val(REDV),使涂层具有内皮细胞选择性.核磁、紫外吸收、红外光谱表征证实聚合物的组成以及REDV多肽在表面的固定;并通过血浆复钙化实验表征涂层的血液相容性.细胞黏附与增殖实验反映REDV多肽构建的涂层表面具备良好的特异性识别并结合内皮细胞的能力.

CONSTRUCTION OF BIOMIMETIC POLYMER SURFACE FOR ENDOTHELIAL CELL SELECTIVITY

This paper aimed to describe a bioactive polymer biointerface for endothelial cells specifically recognition.In particular,biomembrane mimetic polymers with a phospholipid group were designed.A phospholipid polymer-poly2-methacryloyloxyethyl phosphorylcholine(MPC)-co-n-stearyl methacrylate(SMA)-co-p-nitrophenyloxycarbonyl poly(ethylene glycol) methacrylate(MEONP)](PMSN) was synthesized using AIBN as an initiator and coated on substrate(PET) by solvent evaporation.MPC unit could reduce nonspecific adsorption such as platelet,protein and cells.MEONP unit contains active ester groups on the side chains for immobilization of antibody or peptide sch as REDV.The peptide sequence Arg-Glu-Asp-Val(REDV) was known to bind preferentially to endothelial cells.As a result,MEONP units could conjugate REDV and enhance the specific endothelial cells adsorption.HNMR,UV and FTIR spectrum were used to characterize the composition of polymer and immobilization of REDV peptide plasma recaleification time(PRT) was detected to show the hemocompatibility of polymer coating.Cell adhesion and proliferation experiments showed excellent specific recognition of endothelia cells on biomimetic coatings.Many endothelial cells were observed and the number of endothelial cells adhering to the PMSN polymer surfaces increased but cell adhesion was markedly reduced on poly(MPC-co-SMA)(PMS).For smooth muscle cells(SMC),many adherent cells were observed and well spread on PET but little on PMS and PMSN by the reason of nonspecific adhesion of MPC for smooth muscle cells.In conclusion,MEONP units and MPC units of PMSN are important for supporting ECs and for reducing SMCs.The PMSN-coated surface was suitable for biorecognition and preservation of cell function.The biomembrane mimetic phospholipid polymer produced an excellent prospect in cardiovascular stent coating.