Determination of benzhexol hydrochloride by capillary zone electrophoresis with an end-column electrochemiluminescence detection |
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Authors: | Jilin Yan Jifeng Liu Weidong Cao Xiuhua Sun Xiurong Yang Erkang Wang |
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Affiliation: | State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China |
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Abstract: | A capillary zone electrophoresis with end-column electrochemiluminescence (ECL) detector was described for the determination of benzhexol hydrochloride. The detection was based on the tris(2,2′-bypyridine)ruthenium(II) [Ru(bpy)32+] ECL reaction with the analyte. Electrophoresis was performed using a 25 μm i.d. uncoated capillary. 10 mM sodium phosphate buffer (pH=8.0) was used as the running buffer. The solution in the detection cell was 80 mM sodium phosphate (pH=8.0) and 5 mM Ru(bpy)32+. A linear calibration curve of three-orders of magnitude was obtained (with a correlation coefficient of >0.999) from 1.0×10−8 to 1.0×10−5 M and the limit of detection was 6.7×10−9 M (S/N=3). This just provides an easy and sensitive method to determine the active ingredient in pharmaceutical formulations. |
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Keywords: | Author Keywords: Capillary electrophoresis Electrochemiluminescence Benzhexol hydrochloride |
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