铜绿微囊藻强化降解金霉素及降解产物的毒性评估

研究了铜绿微囊藻在模拟自然条件下对金霉素降解的强化作用,并对降解产物进行了毒性评估。结果表明,铜绿微囊藻存在下,浓度为10μmol/L的金霉素在24h内的降解效率可提高42. 27%,主要降解产物为4-差相金霉素、异金霉素和4-差相异金霉素。铜绿微囊藻的生长在强化金霉素降解的过程中受到了抑制,最大抑制率为67. 58%,当金霉素被强化降解后,铜绿微囊藻的生长抑制率降低4. 74%。强化降解后的金霉素对铜绿微囊藻叶绿素和可溶性蛋白含量的负面影响明显减弱,活性氧自由基以及抗氧化系统防御水平降至正常水平。

Enhanced degradation and detoxification of chlortetracycline by Microcystis aeruginosa

The feasibility of the enhanced degradation of chlortetracycline( CTC) by Microcystis aeruginosa was studied,and the toxicity of degradation products was evaluated. As shown by the results,the degradation of CTC( 10 μmol/L) increased 42. 27% in the presence of Microcystis aeruginosa in 24 h. The main degradation products were 4-epi-chlortetracycline, iso-chlortetracycline, and 4-epi-iso-chlortetracycline. The growth of Microcystis aeruginosa was inhibited during the enhancement degradation of CTC,and the highest inhibition rate was 67. 58%. Subsequently,the toxicity of the degradation products was significantly reduced when the CTC was degraded by Microcystis aeruginosa,with the growth inhibition decreased to 4. 74%. In addition,the toxicity evaluation showed that the negative effects on the chlorophyll a and soluble protein content of Microcystis aeruginosa were significantly reduced or even disappeared after enhanced the enhancement degradation treatment,while the generation of reactive oxygen species( ROS) and antioxidant response in algae cells returned to normal levels.