纳米银修饰电极差分脉冲伏安法测定盐酸金霉素

建立了快速测定盐酸金霉素(CTC)的方法。通过NaBH4还原法制备纳米银(AgNPs)溶胶,并利用X射线衍射和紫外-可见光谱进行表征。将制备好的AgNPs滴涂到玻碳电极表面制备修饰电极(AgNPs/GCE),研究了CTC在AgNPs/GCE上的电化学行为及伏安法测定,优化了缓冲溶液和pH等检测条件。结果表明,CTC在pH 3.3的柠檬酸-NaOH-HCl缓冲溶液中检测效果最佳。CTC在AgNPs/GCE上发生2个电子和2个质子的不可逆电化学氧化反应,且反应受吸附控制。最佳条件下,CTC的氧化峰电流与其浓度呈现良好的线性关系,线性范围为0.5~100μmol/L,检出限为0.14μmol/L。该修饰电极可用于河水样品检测。

Determination of chlortetracycline hydrochloride by differential pulse voltammetry with silver nanoparticles modified electrode

In this work,a rapid method for the determination of chlortetracycline hydrochloride(CTC)was developed.Ag nanoparticles(AgNPs)sols were prepared using sodium borohydride as reduction agent and characterized by X-ray diffraction and UV-vis spectroscopy.The prepared AgNPs were immobilized onto the surface of glassy carbon electrode to obtain modified electrode(AgNPs/GCE).The electrochemical behavior and voltammetric determination of CTC on AgNPs/GCE were studied.The detection conditions such as buffer solution and pH were optimized.The best results for CTC were obtained in pH 3.3 citric acid-sodium hydroxidehydrochloric acid buffer solution.CTC underwent an irreversible electrochemical oxidation reaction of two electrons and two protons on AgNPs/GCE,and the reaction was controlled by adsorption.Under the optimal conditions,the oxidation peak current of CTC showed a good linear relationship with its concentration in the range of 0.5-100μmol/L,and the limit of detection was 0.14μmol/L.The modified electrode had been applied to the determination of CTC in river samples with satisfactory results.