柱前衍生化-HPLC法测定恩施3种富硒蔬菜中硒氨基酸

采用HPLC与ICP-MS间隙联用的方法,以柱前衍生化-HPLC法进行定性定量分析,使用ICP-MS鉴定,建立一种富硒蔬菜中硒氨基酸的分离检测方法。结果表明:所测定的硒代氨基酸在其线性范围内呈现良好的线性关系(R2>0.999)。该方法中硒代蛋氨酸和硒代胱氨酸的检出限分别为0.204 mg/L和0.680 mg/L,加标回收率分别为97.4%和94.0%,RSD分别为2.1%和0.69%。检测恩施富硒蔬菜样品,白菜、萝卜叶和苋菜含有硒代蛋氨酸和硒代胱氨酸。此方法可用于富硒蔬菜中硒代氨基酸的含量检测。

Determination of selenium amino acids in Enshi three kinds of selenium enriched vegetables by pre-column derivatization-HPLC

High performance liquid chromatography(HPLC)gap combined with ICP-MS qualitative and quantitative analysis was performed by pre-column derivatization HPLC and identified by ICP-MS to establish a new separation and detection method for selenium amino acids in selenium-enriched vegetables.The determined selenoamino acid showed a good linear relationship in the linear range(R2>0.999).The detection limit(LOD)of selenomethionine was 0.204 mg/L,and LOD of selenocystine was 0.680 mg/L.The spiked recoveries of selenomethionine and selenocystine were 97.4%and 94.0%,RSDs were 2.1%and 0.69%,respectively.Among the tested Enshi selenium-enriched vegetable samples,cabbage,radish leaves and leeks contain selenomethionine and selenocystine.This method can be used to determine the content of selenoamino acids in selenium-enriched vegetables.