Simultaneous analysis of seven 2‐hydroxy fatty acids as tert‐butyldimethylsilyl derivatives in plasma by gas chromatography–mass spectrometry |
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| Authors: | Chan Seo Jaehwan Yoon Yumie Rhee Jong‐Jin Kim Sang‐Jip Nam Wonjae Lee Gwang Lee Sung‐Tae Yee Man‐Jeong Paik |
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| Institution: | 1. College of Pharmacy, Sunchon National University, Suncheon, Republic of Korea;2. Department of Molecular Science and Technology, Ajou University, Suwon, Republic of Korea;3. Department of Internal Medicine, Severance Hospital, Endocrine Research Institute, Yonsei University College of Medicine, Seoul, Republic of Korea;4. College of Biology, Sunchon National University, Suncheon, Republic of Korea;5. Department of Chemistry and Nano Science, Global Top 5 program, Ewha Womans University, Seoul, Republic of Korea;6. College of Pharmacy, Chosun University, Gwangju, Republic of Korea;7. Department of Biomedical Sciences, Ajou University School of Medicine, Suwon, Republic of Korea;8. Department of Physiology, Ajou University School of Medicine, Suwon, Republic of Korea |
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| Abstract: | An efficient method for the simultaneous analysis of seven 2‐hydroxy fatty acids (2‐HFAs) as tert‐butyldimethylsilyl (TBDMS) derivative was developed by gas chromatography–mass spectrometry in selected ion monitoring mode. New mass spectral data on 2‐hydroxycapric, 2‐hydroxypalmitic, 2‐hydroxystearic and 2‐hydroxybehenic acids as di‐TBDMS derivatives for hydroxyl and carboxyl groups were built. Under the optimal conditions, the present method showed a good correlation coefficient (r ≥ 0.999) in the range of 0.01–0.5 µg. The precision showed low relative standard deviation of <10%, and the accuracy (percentage relative error) varied from ?5.2 to 0.3 for the seven 2‐HFAs studied. Recovery rates of all 2‐HFAs were ≥ 93.2% with good precision. When applied to normal human plasma, seven 2‐HFAs were positively identified. Therefore, the present efficient method will be useful for simultaneous analysis of 2‐HFAs in plasma. Copyright © 2014 John Wiley & Sons, Ltd. |
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| Keywords: | 2‐hydroxy fatty acid tert‐butyldimethylsilylation gas chromatography– mass spectrometry plasma |
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