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| 化学改性琼脂糖凝胶的物化性能及其对重组人血白蛋白的纯化应用研究 | |
| 引用本文： | 赵彦鼎,杨博,张鹏宇,王燕,贺小艳,郭立安,贺浪冲.化学改性琼脂糖凝胶的物化性能及其对重组人血白蛋白的纯化应用研究[J].离子交换与吸附,2009,25(3). |
| 作者姓名： | 赵彦鼎 杨博 张鹏宇 王燕 贺小艳 郭立安 贺浪冲 |
| 作者单位： | 1. 西安交大保赛生物技术股份有限公司,西安,710054;西安交通大学天然药物研究与工程中心,西安,710061 2. 西安交大保赛生物技术股份有限公司,西安,710054 3. 西安交通大学天然药物研究与工程中心,西安,710061 |
| 基金项目： | 国家科技部创新基金，国家级火炬计划项目 |
| 摘 要： | 考察了几种经过化学改性后带有离子交换配基的琼脂糖凝胶介质的理化性能.通过外形观察、元素及金属含量分析、柱效测定、耐压及流速测定、离子交换容量和蛋白吸附容量测定,从多方面对性能进行验证比较,并对其在分离纯化重组人血白蛋白的应用性能进行了初步研究.结果表明国产产品与进口产品理化性能相当. |
| 关 键 词： | 琼脂糖凝胶 理化性能 重组人血白蛋白 分离与纯化 |
PHYSICOCHEMICAL PROPERTIES OF MODIFICATION AGAROSE GELS AND THE PERFORMANCE FOR PURIFICATION OF RECOMBINATION ALBUMIN |
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| ZHAO Yanding,ZHANG Pengyu,YANG Bo,WANG Yan,HE Xiaoyan,GUO Lian,HE Langchong.PHYSICOCHEMICAL PROPERTIES OF MODIFICATION AGAROSE GELS AND THE PERFORMANCE FOR PURIFICATION OF RECOMBINATION ALBUMIN[J].Ion Exchange and Adsorption,2009,25(3). | |
| Authors: | ZHAO Yanding ZHANG Pengyu YANG Bo WANG Yan HE Xiaoyan GUO Lian HE Langchong |
| Institution: | ZHAO Y,ing1,2 ZHANG Pengyu2 YANG Bo1 WANG Yan1 HE Xiaoyan1 GUO Lian1,2 HE Langchong2 1.Bio-sep Bio-technique stock Co.,Ltd,Xi'an Jiaotong University,xi'an 710054,China 2.Research & Engineering Center for Natural Medicine Xi'an Jiaotong University,Xi'an 710061,China |
| Abstract: | The physicochemical properties of some agarose gels, which were taken ion exchange functional group by chemical modification, were researched in respects of particle shape, particle size distribution, elemental and metal levels, column efficiency, back-pressure, flow-rate and the exchange capacity, the adsorption capacity, and having a preliminary study on purification of recombination albumin.The results showed that no obvious difference was observed between the national products and the external products. |
| Keywords: | Agarose gel Physicochemical properties Recombination albumin Separation and purification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |